Enzyme Kinetics by Isothermal Titration Calorimetry: Allostery, Inhibition, and Dynamics
نویسندگان
چکیده
منابع مشابه
Enzyme kinetics determined by single-injection isothermal titration calorimetry.
The purposes of this paper are (a) to examine the effect of calorimeter time constant (τ) on heat rate data from a single enzyme injection into substrate in an isothermal titration calorimeter (ITC), (b) to provide information that can be used to predict the optimum experimental conditions for determining the rate constant (k2), Michaelis constant (KM), and enthalpy change of the reaction (ΔRH)...
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ژورنال
عنوان ژورنال: Frontiers in Molecular Biosciences
سال: 2020
ISSN: 2296-889X
DOI: 10.3389/fmolb.2020.583826